Histological characterization of oocyte developmental stages of suruvi Steindachneridion scriptum kept in captivity

Autores

  • Mariana Roza Abreu Universidade Federal de Santa Catarina
  • Patrícia Garcia Universidade Federal de Santa Catarina
  • Evoy Zaniboni-Filho Universidade Federal de Santa Catarina

DOI:

https://doi.org/10.4025/actascianimsci.v37i4.27570

Palavras-chave:

histology, oogenesis, fish reproduction, vitellogenesis.

Resumo

Stages of oocyte development were described for suruvi females (Steindachneridion scriptum) kept in captivity. Oocytes of 21 mature or maturing females and ovaries of 12 immature females were fixed in Karnovsky solution for 4 and 12 hours, respectively. After, they were processed for routine histology, embedded in paraffin and stained with hematoxylin-eosin. Slides were examined under a light microscope and the oocytes were measured using the LAS EZ software, using the average between the largest and the smallest diameter of each oocyte for representation. There were six types of oocytes: Chromatin nucleolus: found in immature females, with average diameter of 9.5 ± 4.46 μm; Perinucleolar: present in all developmental stages with average diameter of 67.6 ± 15.61 μm; Cortical alveolar: measuring 175.6 ± 56.58 μm; Vitellogenic: average diameter of 797.65 ± 136.10 μm; Mature: with average diameter of 1184.98 ± 171.13 μm and Atretic, which were found in mature or maturing females of suruvi.

 

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Biografia do Autor

  • Mariana Roza Abreu, Universidade Federal de Santa Catarina
    Bolsista CNPq no Programa de Pós-Graduação em Aquicultura da Universidade Federal de Santa Catarina – UFSC.
  • Patrícia Garcia, Universidade Federal de Santa Catarina
    Pesquisador AQI/CCA/UFSC. Laboratório AQUOS - Sanidade de Organismos Aquáticos, AQI – CCA -  UFSC. Rod. Admar Gonzaga, 1346 CEP 88040-900, Itacorubi, Florianópolis, SC, Brasil
  • Evoy Zaniboni-Filho, Universidade Federal de Santa Catarina
    Professor AQI/CCA/UFSC. Laboratório de Biologia e Cultivo de Peixes de Ãgua Doce (LAPAD-UFSC). Rodovia SC 406, 3532 – 88066-000 – Lagoa do Peri, Florianópolis, SC.

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Publicado

2015-10-01

Edição

Seção

Aquicultura

Como Citar

Histological characterization of oocyte developmental stages of suruvi Steindachneridion scriptum kept in captivity. (2015). Acta Scientiarum. Animal Sciences, 37(4), 351-356. https://doi.org/10.4025/actascianimsci.v37i4.27570