Influence of Tween on constitutive and inducible laccase production by Botryosphaeria sp - DOI: 10.4025/actascibiolsci.v26i4.1536

Abstract

Botryosphaeria sp. is a ligninolytic fungus that produces laccase. It was previously established that veratryl alcohol (VA) strongly induced laccase production in this ascomyceteous. It is well known that surfactants such as Tween - 80 can increase the production of some fungal exo-enzymes. It has been reported that Tween-80 can also induce laccase production in basidiomycetes. In this work, the influence of Tweens 20, 40, 60 and 80 on the production of constitutive laccases (in the absence of VA) and inducible laccases (in the presence of VA) by Botryosphaeria sp. was evaluated, separately. Laccase activity was assayed using two putative laccase substrates: ABTS (PPO - I) and DMP (PPO - II). All of the Tweens examined increased the production of both constitutive and inducible laccase PPO - I, but did not affect the production of constitutive laccase PPO - II. Tweens 60 and 20 increased the production of inducible laccase PPO - II, while Tween 80 and 40 did not cause any effect