Substrato difusor de nutrientes (SDN): avaliação do método em laboratório para experimentos in situ com perifíton - DOI: 10.4025/actascibiolsci.v26i3.1539

Fabiana Schumacher Fermino; Denise de Campos Bicudo; Cacilda Thais Janson Mercante

doi:10.4025/actascibiolsci.v26i3.1539

**Nutrient-diffusing substrate (NDS): methodological evaluation in laboratory for in situ experiments with periphyton** - DOI: 10.4025/actascibiolsci.v26i3.1539

Authors

Fabiana Schumacher Fermino Instituto de Botânica de São Paulo Author
Denise de Campos Bicudo Instituto de Botânica, Secretaria do Meio Ambiente Author
Cacilda Thais Janson Mercante Instituto de Pesca, SP Author

DOI:

https://doi.org/10.4025/actascibiolsci.v26i3.1539

Keywords:

enriquecimento, difusão, nitrogênio, fósforo, metodologia, perifíton

Abstract

This study evaluated the nutrient-diffusing substrate method in laboratory under controlled temperature (20oC), for further application in in situ bioassay enrichment experiments. The experimental unit comprised a 300mL polystyrene pot filled up with 2% agar solution with isolated or combined addition of nitrogen (0.75 M NaNO₃) and phosphorus (0.05 M KH₂PO₄). The topside opening of the pot was covered with plankton net cloth used for periphyton growth. Nutrients daily release rates were determined during 33 days. Diffusion coefficients obtained by regression analysis were similar in all treatments (0.05day^-1). Results indicated that nutrient diffusion was not influenced by isolated or combined addition, thus allowing comparison between the treatments. In addition, after 4 days N:P diffusing ratio was kept close to the previously established condition. The proposed method has a potential use for nutrient limitation assessments in tropical aquatic ecosystems

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Author Biography

Denise de Campos Bicudo, Instituto de Botânica, Secretaria do Meio Ambiente

Atualmente é Pesquisador Científico VI do Instituto de Botânica da Secretaria do Meio Ambiente de São Paulo. Tem experiência na área de Ecologia com ênfase em Limnologia e na área de Botânica, com ênfase em taxonomia de criptógamos, atuando principalmente nos seguintes temas: eutrofização, ecologia de perifíton, bioindicação, limitação de nutrientres, biodiversidade de algas. Currículo Lattes

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Published

2008-04-01

Issue

Vol. 26 No. 3 (2004)

Section

Biology Sciences

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How to Cite

Nutrient-diffusing substrate (NDS): methodological evaluation in laboratory for in situ experiments with periphyton - DOI: 10.4025/actascibiolsci.v26i3.1539. (2008). Acta Scientiarum. Biological Sciences, 26(3), 273-280. https://doi.org/10.4025/actascibiolsci.v26i3.1539