Cultura de calos de Aspidosperma ramiflorum Muell. Arg.: crescimento e produção de alcalóides

Arildo José Braz de Oliveira; Luzia Koike; Francisco de Assis Machado Reis; Simone Liliane Kirszenzaft Shepherd

doi:10.4025/actascibiolsci.v23i0.2719

Callus culture of Aspidosperma ramiflorum Muell. Arg.: growth and alkaloid production

Authors

Arildo José Braz de Oliveira UEM Author
Luzia Koike UNICAMP Author
Francisco de Assis Machado Reis UNICAMP Author
Simone Liliane Kirszenzaft Shepherd UNICAMP Author

DOI:

https://doi.org/10.4025/actascibiolsci.v23i0.2719

Keywords:

Apocynaceae, Aspidosperma ramiflorum, alcalóides monoterpenóides, ramiflorina A, ramiflorina B, cultura de tecidos

Abstract

Callus culture of Aspidosperma ramiflorum was established in Murashige and Skoog medium. Callus were supplemented with 1 mg.L^-1 2,4-dichlorophenoxyacetic acid, 1.5 mg.L^-1 benzylaminopurine, 30 g L^-1 sucrose and 10 g L^-1 agar. Cultures accumulated the same major alkaloids, ramiflorine A and ramiflorine B, present in the stem bark of the parent plant. The alkaloid contents of 10-methoxy-geissoschizol, ramiflorine A and ramiflorine B, of bark and callus cultures were quantitatively compared by HPLC

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Author Biography

Arildo José Braz de Oliveira, UEM

possui graduação em Farmácia Bioquímica pela Universidade Estadual de Maringá (1987) , mestrado em Química pela Universidade Estadual de Campinas (1994) e doutorado em Química pela Universidade Estadual de Campinas (1999) . Atualmente é Professor adjunto da Universidade Estadual de Maringá, Revisor de periódico da Ciência Rural, Revisor de periódico da Química Nova, Revisor de periódico da Brazilian Archives of Biology and Technology e Revisor de periódico da Acta Horticulturae. Tem experiência na área de Química , com ênfase em Química Orgânica. Atuando principalmente nos seguintes temas: Cromatografia gasosa, alcalóides, biotecnologia, produtos naturais, Aspidosperma e Apocynaceae Currículo Lattes

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Published

2008-05-09

Issue

Vol. 23 (2001)

Section

Biology Sciences

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How to Cite

Callus culture of Aspidosperma ramiflorum Muell. Arg.: growth and alkaloid production. (2008). Acta Scientiarum. Biological Sciences, 23, 609-612. https://doi.org/10.4025/actascibiolsci.v23i0.2719