Utilização da serologia na identificação de Ascia monuste orseis (Latreille, 1819) (Lepidoptera: Pieridae) - DOI: 10.4025/actascibiolsci.v31i2.515

Fabiano de Mello Costa; Ana Paula da Silva Loffredo; Helena Caroline Onody; Carlos Roberto Sousa-Silva

doi:10.4025/actascibiolsci.v31i2.515

**Using the serological technique to identify Ascia monuste orseis (Latreille, 1819) (Lepidoptera: Pieridae)** - DOI: 10.4025/actascibiolsci.v31i2.515

Authors

Fabiano de Mello Costa UFSCAR Author
Ana Paula da Silva Loffredo UFSCAR Author
Helena Caroline Onody UFSCAR Author
Carlos Roberto Sousa-Silva UFSCAR Author

DOI:

https://doi.org/10.4025/actascibiolsci.v31i2.515

Keywords:

Insecta, biological control, antiserum, predation

Abstract

This study aimed to obtain specific antiserum for Ascia monuste orseis (Latreille, 1819), with the aim of identifying the predators associated with this insect. The preparation of specific antiserum was performed by immunizing a rabbit via a lymph nodule injection containing antigen obtained from the maceration of 4^th and 5^th instars of A. monuste orseis caterpillars. Serological homologous tests performed using double diffusion in agar gel were positive 21 days after the first antigen inoculation. Eggs, 1^th, 2^th and 3^th instar caterpillars, pupae and adults of A. monuste orseis also reacted positively to the obtained antiserum.

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Author Biography

Helena Caroline Onody, UFSCAR

Tem experiência na área de Zoologia, com ênfase em Entomologia, atuando principalmente nos seguintes temas: taxonomia e ecologia de Hymenoptera Parasitóides em áreas naturais e agroecossistemas, estudo das interações parasitóide-hospedeiro e avaliação dos efeitos de extratos vegetais em pragas agrícolas Currículo Lattes

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Published

2009-05-14

Issue

Vol. 31 No. 2 (2009)

Section

Ecology and Limnology

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How to Cite

Using the serological technique to identify Ascia monuste orseis (Latreille, 1819) (Lepidoptera: Pieridae) - DOI: 10.4025/actascibiolsci.v31i2.515. (2009). Acta Scientiarum. Biological Sciences, 31(2), 149-151. https://doi.org/10.4025/actascibiolsci.v31i2.515