Variabilidade genética em alface (Lactuca sativa L.) e determinação do número ótimo de marcas RAPD para estudos de diversidade molecular - DOI: 10.4025/actasciagron.v25i1.2142

Flávio Dessune Tardin; Antonio Teixeira do Amaral Júnior; Messias Gonzaga Pereira; Maria Celeste Gonçalves Vidigal; Rogério Figueiredo Daher; Carlos Alberto Scapim

doi:10.4025/actasciagron.v25i1.2142

**Genetic diversity and determination of the optimum number of RAPD markers in lettuce (Lactuca sativa L.)** - DOI: 10.4025/actasciagron.v25i1.2142

Authors

Flávio Dessune Tardin UENF Author
Antonio Teixeira do Amaral Júnior UENF Author
Messias Gonzaga Pereira UENF Author
Maria Celeste Gonçalves Vidigal UEM Author
Rogério Figueiredo Daher UENF Author
Carlos Alberto Scapim UEM Author

DOI:

https://doi.org/10.4025/actasciagron.v25i1.2142

Keywords:

Lactuca sativa, diversidade genética, marcadores RAPD

Abstract

Twenty lettuce accessions from the UENF Germplasm Collection were assessed for genetic diversity using RAPD markers. Multivariate statistical techniques were used, such as grouping analyses, using the Tocher and Single Linkage methods. Analysis of 55 polymorphic markers, obtained with 25 primers, showed that there was sufficient variability in the material studied to be exploited in future breeding programs. The ‘BGH 4060’ and ‘Grand Rapids’ accessions were the most dissimilar, while ‘BGH 4325’ and ‘BGH 4326' were the most similar. There was high agreement between ecogeographic origin and molecular similarity. It was further found by the stress statistic (Kruskal, 1964) and by the correlation between number of markers and increase in ideal formation of groups that 50 polymorphic markers is the optimum number for genetic diversity study in the assessed accessions.

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Author Biography

Antonio Teixeira do Amaral Júnior, UENF

Antonio Teixeira do Amaral Junior concluiu o doutorado em Genética e Melhoramento pela Universidade Federal de Viçosa em 1996. Atualmente é Professor Associado I da Universidade Estadual do Norte Fluminense Darcy Ribeiro. Atua na área de Agronomia, com ênfase em Melhoramento de Milho Pipoca. Em suas atividades profissionais interagiu com 174 colaboradores em co-autorias de trabalhos científicos. Em seu currículo Lattes os termos mais freqÃ¼entes na contextualização da produção científica, tecnológica e artístico-cultural são: milho pipoca, seleção recorrente, biometria, biologia molecular e marcadores de DNA Currículo Lattes

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Published

2008-04-23

Issue

Vol. 25 No. 1 (2003)

Section

Agronomy

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I Declare that current article is original and has not been submitted for publication, in part or in whole, to any other national or international journal.

The copyrights belong exclusively to the authors. Published content is licensed under Creative Commons Attribution 4.0 (CC BY 4.0) guidelines, which allows sharing (copy and distribution of the material in any medium or format) and adaptation (remix, transform, and build upon the material) for any purpose, even commercially, under the terms of attribution.

How to Cite

Tardin, F. D., Amaral Júnior, A. T. do, Pereira, M. G., Vidigal, M. C. G., Daher, R. F., & Scapim, C. A. (2008). Genetic diversity and determination of the optimum number of RAPD markers in lettuce (Lactuca sativa L.) - DOI: 10.4025/actasciagron.v25i1.2142. Acta Scientiarum. Agronomy, 25(1), 1-5. https://doi.org/10.4025/actasciagron.v25i1.2142