Padrões de isozimas de malato desidrogenase em população clonal nos cladófilos de Opuntia ficus-indica Mill (Cactaceae) - DOI: 10.4025/actascibiolsci.v25i1.2093

Alexandro Cezar Faleiro; Adriana Gazoli Resende; Maria de Fátima Pires da Silva Machado

doi:10.4025/actascibiolsci.v25i1.2093

**Malate dehydrogenase isozyme patterns in cladophylls of a Opuntia ficus-indica Mill. (Cactaceae) clonal population** - DOI: 10.4025/actascibiolsci.v25i1.2093

Authors

Alexandro Cezar Faleiro UEM Author
Adriana Gazoli Resende UEM Author
Maria de Fátima Pires da Silva Machado Biologia - UEM Author

DOI:

https://doi.org/10.4025/actascibiolsci.v25i1.2093

Keywords:

isozimas, malato desidrogenase, palma, polimorfismo, similaridade genética

Abstract

Malate dehydrogenase (MDH) isozymes were used as biochemical markers to discriminate and cluster cladophylls of plants of one clonal population of the prickly pear, Opuntia ficus-indica (Cactaceae). The isozyme electrophoretic patterns obtained with MDH provided 8 isozymes and 5 different electrophoretic phenotypes. Similarity in cladophylls was estimated using Jaccard’s coefficient. This clonal population studied was founded by only one propagule, and after 50 years, it is likely to have been formed by asexual and sexual propagules. Since that differential expression of MDH isozymes could play a significant role in overall plant cell metabolism, we suggest that the cladophylls of prickly pear that were clustered together showing identity or higher similarity are specially a suitable source for industrial procedures of industrial extraction of commercial interest compounds because a same extraction protocols can be most quickly and easily standardized using genetically uniform materials. Electrophoretic patterns of MDH isozymes can be used as an effective tool for previously determine the genetic similarity in the cladophylls of O. ficus-indica plants

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Author Biography

Maria de Fátima Pires da Silva Machado, Biologia - UEM

Possui graduação em Licenciatura Em Ciências Biológicas pela Universidade de São Paulo (1978) , mestrado em Ciências Biológicas (Biologia Genética) pela Universidade de São Paulo (1982) , doutorado em Ciências Biológicas (Biologia Genética) pela Universidade de São Paulo (1986) e pos-doutorado pelo Instituto de Biologia Celular de Madrid C S I C (1989) . Atualmente é Professor Associado da Universidade Estadual de Maringá, Revisor de periódico da Ciência Rural e Revisor de periódico da Acta Scientiarum (UEM). Tem experiência na área de Genética , com ênfase em Genética Animal. Atuando principalmente nos seguintes temas: G-3-PDH (EC 1.1.1.8), ISOENZIMAS, HIMENOPTEROS. Currículo Lattes

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Published

2008-04-18

Issue

Vol. 25 No. 1 (2003)

Section

Biology Sciences

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How to Cite

Malate dehydrogenase isozyme patterns in cladophylls of a Opuntia ficus-indica Mill. (Cactaceae) clonal population - DOI: 10.4025/actascibiolsci.v25i1.2093. (2008). Acta Scientiarum. Biological Sciences, 25(1), 207-211. https://doi.org/10.4025/actascibiolsci.v25i1.2093