Estudo de oito espécies da familia Anostomidae (Pisces, Characiformes) através da análise de RAPD

Lucimara Chiari; Leda Maria Koelblinger Sodré

doi:10.4025/actascibiolsci.v23i0.2699

Study of eight species of the Anostomidae family (Pisces, Characiformes) by RAPD analysis

Authors

Lucimara Chiari UEL Author
Leda Maria Koelblinger Sodré UEL Author

DOI:

https://doi.org/10.4025/actascibiolsci.v23i0.2699

Keywords:

DNA, polimorfismo, variabilidade genética, RAPD, Anostomidae, peixe

Abstract

Random Amplified Polymorphic DNA (RAPD) techniques were applied to eight Anostomidae species to analyze the genetic variability and genetic similarity within and between species. The results obtained were compared to those from isoenzyme analysis executed for many of the species studied. The proportion of polymorphic loci obtained with 10 primers was over 40%, with the exception of Leporinus amblyhynchus. This is especially important since genetic variability is a population feature both for short term fitness of the individual and for the long term survival of populations. Through subsequent analysis of dendrograms it was possible to discriminate the two Schizodon species from those of the genus Leporinus, even though it was impossible to differentiate L. obtusidens and L. elongates. RAPD analysis indicates that the two species are genetically very similar, supported too by isoenzyme data

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Author Biography

Leda Maria Koelblinger Sodré, UEL

Possui graduação em Bacharelado Em Ciências Biologicas Modalidade Médica pela Universidade Estadual de Londrina (1973), mestrado em Mestrado em Genética pela Faculdade de Medicina de Ribeirão Preto - FMRP-USP (1978) e doutorado em Doutorado em Ciências, área Concentração Genética pela Faculdade de Medicina de Ribeirão Preto - FMRP-USP (1989). Atualmente é professor associado c da Universidade Estadual de Londrina. Tem experiência na área de Genética, com ênfase em Genética Animal, atuando principalmente nos seguintes temas :peixes, marcadores moleculares, estrutura populacional, diversidade genética, variabilidade genetica e polimorfismo Currículo Lattes

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Published

2008-05-09

Issue

Vol. 23 (2001)

Section

Biology Sciences

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How to Cite

Study of eight species of the Anostomidae family (Pisces, Characiformes) by RAPD analysis. (2008). Acta Scientiarum. Biological Sciences, 23, 445-451. https://doi.org/10.4025/actascibiolsci.v23i0.2699