Acute toxicity of copper sulfate and aqueous extract of dried neem leaves on snails (Pomacea canaliculata) - DOI: 10.4025/actascibiolsci.v30i3.3615

Abstract

Snails can become an environmental and economic problem, causing substantial losses. The objective of this work was to estimate the acute toxicity of copper sulfate pentahydrate (CuSO₄.5H₂O) and the aqueous extract of dried neem leaves on snails (P. canaliculata) under laboratory conditions. In order to estimate the lethal concentration 50% (LC (I)_50;96h), snails were exposed to six increasing copper sulfate concentrations (0.0; 0.01; 0.03; 0.05; 0.07 and 0.1 mg L^-1) and six increasing concentrations of aqueous extract of dried neem leaves 0.0; 100; 125; 150; 175 and 200 mL aqueous extract of dried neem leaves L^-1 water, equivalent to (0.0; 1.18; 1.47; 1.77; 2.06; and 2.36 mg azadirachtin L^-1), in triplicate and one control treatment in an entirely random delineation. Estimated LC (I)(_50;96h) of copper sulfate was 0.02 mg copper sulfate L^-1, with a 0.01 mg L^-1 lower limit and a 0.03 mg L^-1 upper limit. Estimated lethal concentration 50% of the aqueous extract of dried neem leaves was 142.75 mL L^-1, equivalent to 1.68 mg L^-1 of azadirachtine, with a 130.89 mL L^-1 (1.54 mg L^-1) low limit and 155.69 mL L^-1 (1.83 mg L^-1) as the upper limit.