Molecular heterogeneity of Malassezia pachydermatis through RAPD-PCR - doi: 10.4025/actascibiolsci.v32i2.4788

Authors

  • Patrícia da Silva Nascente Universidade Federal de pelotas Author
  • Helen Silveira Coimbra Universidade Federal de Pelotas Author
  • Ana Raquel Mano Meinerz Universidade Federal de Pelotas Author
  • Mário Carlos Araújo Meireles Universidade Federal de Pelotas Author
  • João Roberto Braga Mello Universidade Federal do Rio Grande do Sul Author
  • Luiz Filipe Damé Schuch Universidade Federal de Pelotas Author

DOI:

https://doi.org/10.4025/actascibiolsci.v32i2.4788

Keywords:

Malassezia pachydermatis, molecular biology, otitis externa, dogs

Abstract

Several methodologies in molecular biology have been used in the investigation of Malassezia pachydermatis and its differentiation into subtypes. Recent molecular research of this species includes the use of samples isolated from canine otitis externa and dermatitis, as well as from healthy animals, having in view an epidemiological study of the yeast. The aim of this study was to identify molecular differences in M. pachydermatis samples isolated from dogs with otitis externa. The M. pachydermatis strains were analyzed by means of the Random Amplification Primer DNA - Polimerase Chain Reaction (RAPD–PCR) for molecular heterogeneity research. DNA extraction was carried out with phenol-chloroform and the RAPD technique using the AGAATCCGCC primer. A variation was observed in the number and arrangement of the bands among the 49 studied isolates, grouped into nine patterns. Isolate groupings were not found to be related to animal breed, age or sex. It was concluded that M. pachydermatis has differences in its molecular profile, as shown by the molecular technique (RAPD – PCR), which allows isolates to be classified into nine subtypes.

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Author Biographies

  • Patrícia da Silva Nascente, Universidade Federal de pelotas
    Pós Doutoranda da Faculdade de Veterinária da Universidade Federal de Pelotas - Departamento de Veterinária Preventiva
  • Helen Silveira Coimbra, Universidade Federal de Pelotas
    Doutoranda da Faculdade de Veterinária - UFPel
  • Ana Raquel Mano Meinerz, Universidade Federal de Pelotas
    Pós Doutoranda da Faculdade de Veterinária da UFPel
  • Mário Carlos Araújo Meireles, Universidade Federal de Pelotas
    Dr. Prof. Associado de Doenças Infecciosas da Faculdade de Veterinária - UFPel
  • João Roberto Braga Mello, Universidade Federal do Rio Grande do Sul
    Dr. Prof Adjunto da Faculdade de Veterinária da UFRGS
  • Luiz Filipe Damé Schuch, Universidade Federal de Pelotas
    Dr. Professor Assistente de Doenças Infecciosasda Faculdade de Veterinária - UFPel

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Published

2010-05-24

Issue

Vol. 32 No. 2 (2010)

Section

Genetics

License

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How to Cite

Molecular heterogeneity of Malassezia pachydermatis through RAPD-PCR - doi: 10.4025/actascibiolsci.v32i2.4788. (2010). Acta Scientiarum. Biological Sciences, 32(2), 169-173. https://doi.org/10.4025/actascibiolsci.v32i2.4788
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