Propagação in vitro de Oncidium baueri Lindl. (Orchidaceae) sem usode ágar - DOI: 10.4025/actasciagron.v28i1.1672

Ricardo Tadeu de Faria; Ronaldo José Durigan Dalio; Lilian Keiko Unemoto; Geraldo Lopes da Silva

doi:10.4025/actasciagron.v28i1.1672

In vitro orchid propagation of Oncidium baueri (Orchidaceae) without the use of agar - DOI: 10.4025/actasciagron.v28i1.1672

Authors

Ricardo Tadeu de Faria UEL Author
Ronaldo José Durigan Dalio UEL Author
Lilian Keiko Unemoto UEL Author
Geraldo Lopes da Silva UEL Author

DOI:

https://doi.org/10.4025/actasciagron.v28i1.1672

Keywords:

orquídeas, substratos, cultura de tecidos, biofábrica, redução de custos, Oncidium

Abstract

This research aims at studying the in vitro propagation of Oncidium baueri (Orchidaceae) without the use of agar as a solidifying agent of culture medium. The seeds were germinated in Murashige and Skoog medium (MS, 1962) modified with half of the macronutrients concentrations. When the seedlings reach an average height of 1 cm, they were subcultivated in 600 mL plastic flasks containing 200 mL of the same nutritive medium used for germination. The evaluated treatments were: 7 g of agar per liter + 1/2 MS medium liquid; chopped (polyurethane) foam + 1/2 MS medium liquid; sphagnum + 1/2 MS medium liquid; thick sand + 1/2MS medium liquid. The evaluated variables after 8 months of study were: aerial part height, tallest root length, sprout number, root number and total fresh mass. The statistics analysis demonstrates that the sphagnum or thick sand treatments are not indicated to substitute the agar. However, the treatment with chopped polyurethane foam showed an optimum rooting and vegetative development of seedlings, being, therefore, an efficient and less costly alternative to replace the agar in the in vitro propagation of Oncidium baueri.

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Author Biography

Ricardo Tadeu de Faria, UEL

Possui graduação em Agronomia pela Faculdade de Agronomia e Zootecnia Manoel Carlos Gonçalves (1987) , mestrado em Genética e Biologia Molecular pela Universidade Estadual de Campinas (1992) , doutorado em Genética e Biologia Molecular pela Universidade Estadual de Campinas (1998) e pos-doutorado pela Universidade de La Laguna (2003) . Atualmente é Associado A da Universidade Estadual de Londrina, Revisor de periódico da Acta Scientiarum (UEM), Revisor de periódico da Semina Ciência Agrária, Revisor de periódico da Revista Brasileira de Horticultura Ornamental, Revisor de periódico da Horticultura Brasileira, Revisor de periódico da Magistra, Revisor de periódico da Magistra, Revisor de periódico da Crop Breeding and Applied Biotechnology e Revisor de periódico da Ciência e Agrotecnologia. Tem experiência na área de Agronomia , com ênfase em Floricultura, Parques e Jardins Currículo Lattes

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Published

2008-04-04

Issue

Vol. 28 No. 1 (2006)

Section

Crop Production

License

DECLARATION OF ORIGINALITY AND COPYRIGHTS

I Declare that current article is original and has not been submitted for publication, in part or in whole, to any other national or international journal.

The copyrights belong exclusively to the authors. Published content is licensed under Creative Commons Attribution 4.0 (CC BY 4.0) guidelines, which allows sharing (copy and distribution of the material in any medium or format) and adaptation (remix, transform, and build upon the material) for any purpose, even commercially, under the terms of attribution.

How to Cite

Faria, R. T. de, Dalio, R. J. D., Unemoto, L. K., & Silva, G. L. da. (2008). In vitro orchid propagation of Oncidium baueri (Orchidaceae) without the use of agar - DOI: 10.4025/actasciagron.v28i1.1672. Acta Scientiarum. Agronomy, 28(1), 71-74. https://doi.org/10.4025/actasciagron.v28i1.1672