Analysis of indicators of osteogenesis, cytotoxicity and genotoxicity of an experimental β-TCP compared to other bone substitutes

Autores/as

  • Noala Vicensoto Moreira Milhan Universidade Estadual Paulista Autor/a
  • Isabel Chaves Silva Carvalho Universidade Estadual Paulista Autor/a
  • Renata Falchete do Prado Universidade Estadual Paulista Autor/a
  • Eliandra de Sousa Trichês Universidade Federal de São Paulo Autor/a
  • Carlos Henrique Ribeiro Camargo Universidade Estadual Paulista Autor/a
  • Samira Esteves Afonso Camargo Universidade Estadual Paulista Autor/a

DOI:

https://doi.org/10.4025/actascihealthsci.v39i1.29788

Palabras clave:

Beta tri-calcium phosphate, bovine hydroxyapatite.

Resumen

The aim of this study was to evaluate the indicators of osteogenesis, cytotoxicity and genotoxicity of an experimental beta tri-calcium phosphate (experimental β-TCP) compared with two other bone substitutes: bovine hydroxyapatite (HA) (Bio-Oss® - Geistlich) and beta tri-calcium phosphate (β-TCP - Bionnovation). The cell viability and genotoxicity were measured by MTT and MNT assay, respectively. The indicators of osteogenesis were analyzed by alkaline phosphatase activity, total protein content, and calcium deposition. The MTT and MNT assay showed that none of the tested materials was cytotoxic nor genotoxic. Concerning the indicators of osteogenesis, it was observed that cells in contact with all the materials were able to induce the osteogenesis and this process was influenced by the period of the cell culture in contact with bone substitutes. Based on the results of this study, it was concluded that this experimental β-TCP appears to be a promising material as a bone substitute.

 

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Biografía del autor/a

  • Noala Vicensoto Moreira Milhan, Universidade Estadual Paulista
    Departamento de Biociências e Diagnóstico Bucal

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Publicado

2017-05-31

Número

Sección

Odontologia

Cómo citar

Analysis of indicators of osteogenesis, cytotoxicity and genotoxicity of an experimental β-TCP compared to other bone substitutes. (2017). Acta Scientiarum. Health Sciences, 39(1), 97-105. https://doi.org/10.4025/actascihealthsci.v39i1.29788

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