Analysis of indicators of osteogenesis, cytotoxicity and genotoxicity of an experimental Î²-TCP compared to other bone substitutes

Noala Vicensoto Moreira Milhan; Isabel Chaves Silva Carvalho; Renata Falchete do Prado; Eliandra de Sousa Trichês; Carlos Henrique Ribeiro Camargo; Samira Esteves Afonso Camargo

doi:10.4025/actascihealthsci.v39i1.29788

Analysis of indicators of osteogenesis, cytotoxicity and genotoxicity of an experimental Î²-TCP compared to other bone substitutes

Auteurs-es

Noala Vicensoto Moreira Milhan Universidade Estadual Paulista Auteur
Isabel Chaves Silva Carvalho Universidade Estadual Paulista Auteur
Renata Falchete do Prado Universidade Estadual Paulista Auteur
Eliandra de Sousa Trichês Universidade Federal de São Paulo Auteur
Carlos Henrique Ribeiro Camargo Universidade Estadual Paulista Auteur
Samira Esteves Afonso Camargo Universidade Estadual Paulista Auteur

DOI :

https://doi.org/10.4025/actascihealthsci.v39i1.29788

Mots-clés :

Beta tri-calcium phosphate, bovine hydroxyapatite.

Résumé

The aim of this study was to evaluate the indicators of osteogenesis, cytotoxicity and genotoxicity of an experimental beta tri-calcium phosphate (experimental Î²-TCP) compared with two other bone substitutes: bovine hydroxyapatite (HA) (Bio-Oss^® - Geistlich) and beta tri-calcium phosphate (Î²-TCP - Bionnovation). The cell viability and genotoxicity were measured by MTT and MNT assay, respectively. The indicators of osteogenesis were analyzed by alkaline phosphatase activity, total protein content, and calcium deposition. The MTT and MNT assay showed that none of the tested materials was cytotoxic nor genotoxic. Concerning the indicators of osteogenesis, it was observed that cells in contact with all the materials were able to induce the osteogenesis and this process was influenced by the period of the cell culture in contact with bone substitutes. Based on the results of this study, it was concluded that this experimental Î²-TCP appears to be a promising material as a bone substitute.

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Biographie de l'auteur-e

Noala Vicensoto Moreira Milhan, Universidade Estadual Paulista

Departamento de Biociências e Diagnóstico Bucal

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PDF (Anglais)

Publié

2017-05-31

Numéro

Vol. 39 No. 1 (2017)

Rubrique

Odontologia

Licence

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Comment citer

Analysis of indicators of osteogenesis, cytotoxicity and genotoxicity of an experimental Î²-TCP compared to other bone substitutes. (2017). Acta Scientiarum. Health Sciences, 39(1), 97-105. https://doi.org/10.4025/actascihealthsci.v39i1.29788